Hotspot

rpoB-trnC-GCA

LSC · 27151-28395 · 1245 bp

Back to Amsonia

Pi 0.0113

MarkerSeek score 65.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1245
variable_sites: 14
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAAGTTCCTCCGCCAAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1414-1423 bp	1.0	79.8
rpoB-trnC-GCA_p2	TAAAGTTCCTCCGCCAAACC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	58.4	1396-1405 bp	1.0	77.6
rpoB-trnC-GCA_p3	CGCCAAACCCTGATCAATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1403-1412 bp	1.0	76.3
rpoB-trnC-GCA_p4	TAAAGTTCCTCCGCCAAACC	TTTGTTGATCAGGCGACACC	50.0	50.0	58.1	58.8	1433-1442 bp	1.0	74.4
rpoB-trnC-GCA_p5	CGCCAAACCCTGATCAATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	58.4	1385-1394 bp	1.0	74.1