Hotspot

atpH-atpI

LSC · 13756-14894 · 1139 bp

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Pi 0.0169

MarkerSeek score 69.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 19
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1227-1231 bp	1.0	77.9
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1310-1314 bp	1.0	76.9
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	57.9	58.5	1314-1318 bp	1.0	76.9
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1228-1232 bp	1.0	76.7
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	1212-1216 bp	1.0	76.2