Hotspot

psbK-psbI

LSC · 8237-8403 · 167 bp

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Pi 0.0663
MarkerSeek score 77.80
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
167
variable_sites
11
pi
0.0663
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 CTTTTGTTTGGCAAGCTGCT AACCCCCTTATTCTTCACGC 45.0 50.0 58.1 57.9 320 bp 1.0 81.6
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG AACCCCCTTATTCTTCACGC 50.0 50.0 57.9 57.9 315 bp 1.0 81.6
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG CCTTATTCTTCACGCCCAGG 50.0 55.0 57.9 58.3 310 bp 1.0 80.0
psbK-psbI_p4 CTTTTGTTTGGCAAGCTGCT CCCTTATTCTTCACGCCCAG 45.0 55.0 58.1 58.3 316 bp 1.0 79.9
psbK-psbI_p5 CTTTTGTTTGGCAAGCTGCT CCTTATTCTTCACGCCCAGG 45.0 55.0 58.1 58.3 315 bp 1.0 79.9