Hotspot

ccsA

SSC · 106126-107049 · 924 bp

Back to Amphipogon

Pi 0.00974

MarkerSeek score 50.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 924
variable_sites: 9
pi: 0.00974
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ACTCTCCCAATCTCGACGAT	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	1480-2436 bp	1.0	80.6
ccsA_p2	TAGACACGCTGCTCTTAGGA	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	1391-2437 bp	1.0	80.1
ccsA_p3	ACTCTCCCAATCTCGACGAT	CTTGCCAGGAATGAGTGGTT	50.0	50.0	57.9	58.1	1684-2640 bp	1.0	80.1
ccsA_p4	TTAGCCGCCATGGTGAAATT	GGATTCTGGACCACGAGAAC	45.0	55.0	58.2	58.0	1413-2438 bp	1.0	79.8
ccsA_p5	ACTCTCCCAATCTCGACGAT	CGTTCGAGGGGTTCTCAAAT	50.0	50.0	57.9	57.9	1217 bp	0.5	58.8