Hotspot

ndhH-rps15

IRa · 114868-114993 · 126 bp

Back to Amphipogon

Pi 0.0661

MarkerSeek score 79.40

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: IRa
length_bp: 126
variable_sites: 8
pi: 0.0661
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	AACACCATGCATTGATGGGT	GTTTGCTGGCTTATTTGGCA	45.0	45.0	58.1	57.5	281-294 bp	1.0	79.5
ndhH-rps15_p2	TCAGTCGAAGAACACCATGC	GTTTGCTGGCTTATTTGGCA	50.0	45.0	58.2	57.5	291-304 bp	1.0	78.2
ndhH-rps15_p3	ATGCATTGATGGGTGGTGAG	GTTTGCTGGCTTATTTGGCA	50.0	45.0	58.2	57.5	275-288 bp	1.0	78.2
ndhH-rps15_p4	AACACCATGCATTGATGGGT	CGTTTGCTGGCTTATTTGGC	45.0	50.0	58.1	58.7	282-295 bp	1.0	77.9
ndhH-rps15_p5	AACACCATGCATTGATGGGT	ACGTTTGCTGGCTTATTTGG	45.0	45.0	58.1	57.3	283-296 bp	1.0	77.3