Hotspot

ccsA-ndhD

SSC · 107050-107239 · 190 bp

Back to Amphipogon

Pi 0.0842
MarkerSeek score 73.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
190
variable_sites
16
pi
0.0842
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTCAGCACTTGTAGCTTCGA GTAGAGGCTCTCTTATCCAA 45.0 45.0 57.2 53.3 341-2333 bp 1.0 41.0
ccsA-ndhD_p2 TTCAGCACTTGTAGCTTCGA AGAGGCTCTCTTATCCAATT 45.0 40.0 57.2 53.1 339-2331 bp 1.0 41.0
ccsA-ndhD_p3 TCAGCACTTGTAGCTTCGAT GTAGAGGCTCTCTTATCCAA 45.0 45.0 57.0 53.3 340-2332 bp 1.0 41.0
ccsA-ndhD_p4 ACGAATTCAGCACTTGTAGCT GTAGAGGCTCTCTTATCCAA 42.9 45.0 58.0 53.3 346-2333 bp 1.0 41.0
ccsA-ndhD_p5 TTCAGCACTTGTAGCTTCGA GTAGAGGCTCTCTTATCCAAT 45.0 42.9 57.2 53.9 341-2333 bp 1.0 41.0