Hotspot

ndhF-rpl32

SSC · 104432-105285 · 854 bp

Back to Amphipogon

Pi 0.0544

MarkerSeek score 78.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 854
variable_sites: 43
pi: 0.0544
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCCACAAGTTCCC	ATCGTCGAGATTGGGAGAGT	50.0	50.0	58.0	57.9	1081-1958 bp	1.0	86.3
ndhF-rpl32_p2	CACATTCCCACAAGTTCCCA	ATCGTCGAGATTGGGAGAGT	50.0	50.0	58.0	57.9	1080-1957 bp	1.0	86.3
ndhF-rpl32_p3	ACACATTCCCACAAGTTCCC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1080-2047 bp	1.0	85.6
ndhF-rpl32_p4	CACATTCCCACAAGTTCCCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1079-2046 bp	1.0	85.6
ndhF-rpl32_p5	ACACATTCCCACAAGTTCCC	AATTTCACCATGGCGGCTAA	50.0	45.0	58.0	58.2	1080-2025 bp	1.0	85.4