Hotspot

rpl14-rpl16

LSC · 78222-78327 · 106 bp

Back to Amphipogon

Pi 0.0485

MarkerSeek score 72.30

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 106
variable_sites: 5
pi: 0.0485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCAGCAGTTCCTATGACTCG	TAGCTGCCAGTAAAATGCCC	55.0	50.0	57.8	58.2	242-245 bp	1.0	79.0
rpl14-rpl16_p2	CGAGCTCCACTGTTATCTGC	TAGCTGCCAGTAAAATGCCC	55.0	50.0	58.4	58.2	206-209 bp	1.0	77.7
rpl14-rpl16_p3	CCAGCAGTTCCTATGACTCG	GCCAGTAAAATGCCCATACG	55.0	50.0	57.8	57.2	237-240 bp	1.0	74.8
rpl14-rpl16_p4	CGAGCTCCACTGTTATCTGC	GCCAGTAAAATGCCCATACG	55.0	50.0	58.4	57.2	201-204 bp	1.0	73.5
rpl14-rpl16_p5	GAGCTCCACTGTTATCTGCT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	57.0	58.2	205-208 bp	1.0	73.4