Hotspot

rps11-rpl36

LSC · 76528-76700 · 173 bp

Back to Amphipogon

Pi 0.0533

MarkerSeek score 69.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 9
pi: 0.0533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	AAACCCACCGTACGAATAGC	GCGTGGGCGAATTAGAGTAA	50.0	50.0	58.0	58.1	505-509 bp	1.0	82.5
rps11-rpl36_p2	GTGTTTGGGTGGATCCTGTT	GCGTGGGCGAATTAGAGTAA	50.0	50.0	58.0	58.1	760-764 bp	1.0	81.8
rps11-rpl36_p3	AAACCCACCGTACGAATAGC	ATTCGAAGCGTACCGAAGAC	50.0	50.0	58.0	58.1	711-715 bp	1.0	81.7
rps11-rpl36_p4	GTGTTTGGGTGGATCCTGTT	ATTCGAAGCGTACCGAAGAC	50.0	50.0	58.0	58.1	966-970 bp	1.0	81.4
rps11-rpl36_p5	TTGGGTGGATCCTGTTACCT	GCGTGGGCGAATTAGAGTAA	50.0	50.0	57.9	58.1	756-760 bp	1.0	81.4