Hotspot

psbE-petL

LSC · 62988-64038 · 1051 bp

Back to Amphipogon

Pi 0.0236
MarkerSeek score 69.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1051
variable_sites
24
pi
0.0236
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCG 45.0 50.0 58.0 58.5 1209-1321 bp 1.0 79.7
psbE-petL_p2 TGACAAATAACCAACCCGCA GCAGCCAGTAGAAAACCGAA 45.0 50.0 58.0 58.5 1207-1319 bp 1.0 79.6
psbE-petL_p3 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1198-1310 bp 1.0 79.4
psbE-petL_p4 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1196-1308 bp 1.0 79.4
psbE-petL_p5 ACCAACCCGCAATGAATAGG AAGCAGCCAGTAGAAAACCG 50.0 50.0 58.2 58.5 1200-1312 bp 1.0 77.9