Hotspot

rbcL-psaI

LSC · 57131-58018 · 888 bp

Back to Amphipogon

Pi 0.039

MarkerSeek score 68.20

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 888
variable_sites: 34
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAGCCGGTAGATACCATCGA	CAATTGCCGGCAACACTAAG	50.0	50.0	58.0	58.0	964-976 bp	1.0	84.2
rbcL-psaI_p2	GAGCTGTATTTGCGAGGGAA	CAATTGCCGGCAACACTAAG	50.0	50.0	58.0	58.0	1614-1626 bp	1.0	82.7
rbcL-psaI_p3	CGCCGGTACAGTAGTAGGTA	CAATTGCCGGCAACACTAAG	55.0	50.0	58.0	58.0	1391-1403 bp	1.0	82.7
rbcL-psaI_p4	AAGCCGGTAGATACCATCGA	AATCGGTCATAACCACTGCC	50.0	50.0	58.0	58.0	1635-1641 bp	1.0	82.6
rbcL-psaI_p5	GAGCTGTATTTGCGAGGGAA	AATCGGTCATAACCACTGCC	50.0	50.0	58.0	58.0	2285-2291 bp	1.0	82.0