Hotspot

ndhC-trnV-UAC

LSC · 51021-51930 · 910 bp

Back to Amphipogon

Pi 0.0291
MarkerSeek score 66.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
910
variable_sites
26
pi
0.0291
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 36.4 55.0 56.1 57.9 952-955 bp 1.0 51.9
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 36.4 55.0 56.1 58.3 953-956 bp 1.0 50.1
ndhC-trnV-UAC_p3 GATCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 37.5 55.0 57.7 57.9 954-957 bp 1.0 48.2
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 38.1 55.0 54.6 57.9 951-954 bp 1.0 47.9
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 952-955 bp 1.0 47.9