Hotspot

rps14-psaB

LSC · 38029-38182 · 154 bp

Back to Amphipogon

Pi 0.0458

MarkerSeek score 79.40

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 7
pi: 0.0458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	ATTTCTGCCGCTTCTTCTCC	GCTCACTTTTCCGTGGGTTA	50.0	50.0	58.3	58.1	275 bp	1.0	79.4
rps14-psaB_p2	ATTTCTGCCGCTTCTTCTCC	TTAGCTCACTTTTCCGTGGG	50.0	50.0	58.3	57.8	278 bp	1.0	78.8
rps14-psaB_p3	ATTTCTGCCGCTTCTTCTCC	GCAGCTTTCTTGATTGCCTC	50.0	50.0	58.3	57.7	242 bp	1.0	78.4
rps14-psaB_p4	ATTTCTGCCGCTTCTTCTCC	GCTCTTTCCATTGTGCAAGC	50.0	50.0	58.3	58.3	311 bp	1.0	77.7
rps14-psaB_p5	ATTTCTGCCGCTTCTTCTCC	TGGCTCTTTCCATTGTGCAA	50.0	45.0	58.3	58.3	313 bp	1.0	77.6