Hotspot

trnD-GUC-psbM

LSC · 16701-17749 · 1049 bp

Back to Amphipogon

Pi 0.0417

MarkerSeek score 61.80

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 23
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.53
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCA	50.0	40.0	58.5	54.5	676-1134 bp	1.0	54.1
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	ATGCGAGAATATTGACTTCCA	50.0	38.1	58.5	55.1	677-1135 bp	1.0	51.0
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCAT	50.0	38.1	58.5	55.1	676-1134 bp	1.0	51.0
trnD-GUC-psbM_p4	GTTCAATTGGTCAGAGCACC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.0	54.5	677-1135 bp	1.0	49.9
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	AATGCGAGAATATTGACTTCCA	50.0	36.4	58.5	55.9	678-1136 bp	1.0	49.2