Hotspot

rpoA

IRb · 77315-78388 · 1074 bp

Back to Amphilophium

Pi 0.11

MarkerSeek score 77.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: IRb
length_bp: 1074
variable_sites: 315
pi: 0.11
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GGGCTTAGCGGTTCTAGAAC	CCGAGAACTGGTTCACGTAG	55.0	55.0	58.1	58.0	2018-2191 bp	1.0	91.3
rpoA_p2	GGGCTTAGCGGTTCTAGAAC	GGAAGAGACGCAGCATTACA	55.0	50.0	58.1	58.0	1709-1858 bp	0.6	75.4
rpoA_p3	TTGGTTGGGTATTGGTGCAA	GGAAGAGACGCAGCATTACA	45.0	50.0	57.9	58.0	1442-1591 bp	0.6	75.2
rpoA_p4	GGGCTTAGCGGTTCTAGAAC	ATTCGCCGAAGTCGTATACG	55.0	50.0	58.1	58.0	1776-1781 bp	0.2	48.7
rpoA_p5	GGGCTTAGCGGTTCTAGAAC	GCAACGAGCAGGAGTTATGA	55.0	50.0	58.1	58.0	1837 bp	0.1	43.4