Hotspot

ndhG-ndhI

SSC · 122192-122563 · 372 bp

Back to Amphilophium

Pi 0.0302

MarkerSeek score 72.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 372
variable_sites: 34
pi: 0.0302
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	CAATTTCTTTGAATTCACCTCA	50.0	31.8	58.0	53.2	432-472 bp	1.0	49.9
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	CAATTTCTTTGAATTCACCTCAA	50.0	30.4	58.0	54.0	432-472 bp	1.0	49.9
ndhG-ndhI_p3	AAGACCCGATCCCAGAAAGA	CAATTTCTTTGAATTCACCTCAAA	50.0	29.2	58.0	54.8	432-472 bp	1.0	49.9
ndhG-ndhI_p4	ACCCGATCCCAGAAAGACTA	CAATTTCTTTGAATTCACCTCA	50.0	31.8	57.5	53.2	429-469 bp	1.0	49.9
ndhG-ndhI_p5	ACCCGATCCCAGAAAGACTA	CAATTTCTTTGAATTCACCTCAA	50.0	30.4	57.5	54.0	429-469 bp	1.0	49.9