Hotspot

rpoA-rps11

IRb · 78389-78443 · 55 bp

Back to Amphilophium

Pi 0.36

MarkerSeek score 72.00

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: IRb
length_bp: 55
variable_sites: 40
pi: 0.36
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	CAAGTGCTTCTACCGGAGTC	CCGAGAACTGGTTCACGTAG	55.0	55.0	58.0	58.0	1137-1304 bp	1.0	91.8
rpoA-rps11_p2	CAAGTGCTTCTACCGGAGTC	GGAAGAGACGCAGCATTACA	55.0	50.0	58.0	58.0	828-971 bp	0.6	75.9
rpoA-rps11_p3	CAAGTGCTTCTACCGGAGTC	ATTCGCCGAAGTCGTATACG	55.0	50.0	58.0	58.0	866 bp	0.2	50.2
rpoA-rps11_p4	AACGCATCTCCACTGTGTTT	GGAAGAGACGCAGCATTACA	45.0	50.0	58.0	58.0	226 bp	0.2	49.1
rpoA-rps11_p5	CAAGTGCTTCTACCGGAGTC	GCAACGAGCAGGAGTTATGA	55.0	50.0	58.0	58.0	927 bp	0.1	43.9