Hotspot

rbcL-accD

LSC · 56335-57113 · 779 bp

Back to Amphilophium

Pi 0.0322

MarkerSeek score 75.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 75
pi: 0.0322
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACTACAGTTCGGTGGAGGAA	AGAACCCACAAAGACGCAAT	50.0	45.0	58.0	58.0	1309-1511 bp	1.0	90.6
rbcL-accD_p2	ACTACAGTTCGGTGGAGGAA	TTCTTCCGAATCGCCTCTTG	50.0	50.0	58.0	58.0	1615-1616 bp	0.2	48.2
rbcL-accD_p3	GGAGTCTTGAACTAGCTGCC	TTCTTCCGAATCGCCTCTTG	55.0	50.0	58.0	58.0	1460-1461 bp	0.2	48.2
rbcL-accD_p4	TTGAATGCTACTGCGGGTAC	TTCTTCCGAATCGCCTCTTG	50.0	50.0	58.0	58.0	2094-2095 bp	0.2	48.1
rbcL-accD_p5	ACTACAGTTCGGTGGAGGAA	TTGGTGTGTCATATCCTGCG	50.0	50.0	58.0	58.0	1146-1147 bp	0.2	47.8