Hotspot

trnM-CAU-atpE

LSC · 52027-52224 · 198 bp

Back to Amphilophium

Pi 0.0344

MarkerSeek score 71.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 21
pi: 0.0344
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TTTGGTTCGCTTGGGAATGA	GGCAAATCTAGCTCTCCGAC	45.0	55.0	58.0	58.1	356-359 bp	1.0	84.4
trnM-CAU-atpE_p2	TTTGGTTCGCTTGGGAATGA	GAGGCAAATCTAGCTCTCCG	45.0	55.0	58.0	57.9	358-361 bp	1.0	84.3
trnM-CAU-atpE_p3	GTTCCATTTGGTTCGCTTGG	GGCAAATCTAGCTCTCCGAC	50.0	55.0	57.9	58.1	362-365 bp	1.0	83.9
trnM-CAU-atpE_p4	GTTCCATTTGGTTCGCTTGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	57.9	57.9	364-367 bp	1.0	83.8
trnM-CAU-atpE_p5	TTTGGTTCGCTTGGGAATGA	AGGCAAATCTAGCTCTCCGA	45.0	50.0	58.0	58.2	357-360 bp	1.0	83.7