Hotspot

trnS-GCU-trnG-UCC

LSC · 8013-8765 · 753 bp

Back to Amphilophium

Pi 0.0251
MarkerSeek score 70.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
753
variable_sites
51
pi
0.0251
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 751-829 bp 1.0 60.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 750-828 bp 1.0 60.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 752-830 bp 1.0 58.9
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 751-829 bp 1.0 58.9
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC ACGAATCACACTTTTACCACT 50.0 38.1 58.4 55.5 753-831 bp 1.0 58.0