Hotspot

ycf1

SSC · 126412-131829 · 5418 bp

Back to Amphicarpaea

Pi 0.0196

MarkerSeek score 49.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5418
variable_sites: 106
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTTCAAATCAGTTCTAATTGCA	AGTAATTATCCAGAATTAGAGGAA	33.3	29.2	57.4	52.7	236 bp	1.0	43.7
ycf1_p2	TCGTTCAAATCAGTTCTAATTGCA	AGTAATTATCCAGAATTAGAGGAAA	33.3	28.0	57.4	53.5	236 bp	1.0	43.7
ycf1_p3	CGTTCAAATCAGTTCTAATTGCA	AGTAATTATCCAGAATTAGAGGAA	34.8	29.2	56.1	52.7	235 bp	1.0	43.7
ycf1_p4	TCGTTCAAATCAGTTCTAATTGCA	AGTAATTATCCAGAATTAGAGGAAAA	33.3	26.9	57.4	54.2	236 bp	1.0	43.7
ycf1_p5	TTCGTTCAAATCAGTTCTAATTGCA	AGTAATTATCCAGAATTAGAGGAA	32.0	29.2	58.0	52.7	237 bp	1.0	43.7