Hotspot

rps15-ycf1

SSC · 125968-126411 · 444 bp

Back to Amphicarpaea

Pi 0.0276

MarkerSeek score 70.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 444
variable_sites: 12
pi: 0.0276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACCGATCCCGAGTTTTT	ATCCGCGAGTGAAAATTCGT	45.0	45.0	57.4	58.3	535-554 bp	1.0	76.6
rps15-ycf1_p2	TTCAACCGATCCCGAGTTTT	ATCCGCGAGTGAAAATTCGT	45.0	45.0	57.4	58.3	536-555 bp	1.0	76.6
rps15-ycf1_p3	TCAACCGATCCCGAGTTTTT	CAGATGTATCCGCGAGTGAA	45.0	50.0	57.4	57.5	542-561 bp	1.0	74.8
rps15-ycf1_p4	TTCAACCGATCCCGAGTTTT	CAGATGTATCCGCGAGTGAA	45.0	50.0	57.4	57.5	543-562 bp	1.0	74.8
rps15-ycf1_p5	TCAACCGATCCCGAGTTTTT	TCCGCGAGTGAAAATTCGTT	45.0	45.0	57.4	58.5	534-553 bp	1.0	74.8