Hotspot

rps3-rps19

LSC · 87192-87598 · 407 bp

Back to Amphicarpaea

Pi 0.0373

MarkerSeek score 63.30

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 15
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	ACTCCTTTTGTACCCGCTTG	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	1338-1363 bp	1.0	80.2
rps3-rps19_p2	CTCCTTTTGTACCCGCTTGT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	1337-1362 bp	1.0	80.2
rps3-rps19_p3	TGATCCATTCAACACGTGCA	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.1	58.1	1397-1422 bp	1.0	80.1
rps3-rps19_p4	TGAACTCCTTTTGTACCCGC	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	1341-1366 bp	1.0	80.1
rps3-rps19_p5	CTCCTTTTGTACCCGCTTGT	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.1	58.1	1525-1550 bp	1.0	79.6