Hotspot

rpl20-rps12

LSC · 71991-72831 · 841 bp

Back to Amphicarpaea

Pi 0.00957

MarkerSeek score 64.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 8
pi: 0.00957
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTTGTCCTCCGTTTCCGAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.1	57.7	973 bp	1.0	77.9
rpl20-rps12_p2	TTTTGTCCTCCGTTTCCGAG	AAAATCTCCAGCTCTTCGGG	50.0	50.0	58.1	57.6	947 bp	1.0	76.8
rpl20-rps12_p3	AAGTCTTGAATGAGCTCCGC	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.3	57.7	1018 bp	1.0	76.7
rpl20-rps12_p4	TTTTTGTCCTCCGTTTCCGA	ACAAGACAGCCAATCCGAAA	45.0	45.0	57.7	57.7	974 bp	1.0	76.3
rpl20-rps12_p5	TTTTGTCCTCCGTTTCCGAG	CACAAGACAGCCAATCCGAA	50.0	50.0	58.1	58.5	974 bp	1.0	76.2