Hotspot

ycf4-cemA

LSC · 63884-64380 · 497 bp

Back to Amphicarpaea

Pi 0.0202

MarkerSeek score 54.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 10
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGTTTGTTCCCTTGACTCGT	CCCTGGGTTGAATGGAATCC	50.0	55.0	58.0	58.2	1106-1107 bp	1.0	79.0
ycf4-cemA_p2	ATTTCCGGCAATTGCAATGG	CCCTGGGTTGAATGGAATCC	45.0	55.0	58.0	58.2	1748-1749 bp	1.0	78.9
ycf4-cemA_p3	CGTTGGGGATTTCCTGGAAA	CCCTGGGTTGAATGGAATCC	50.0	55.0	58.1	58.2	1263-1264 bp	1.0	78.5
ycf4-cemA_p4	TGGGGTTATTGTTGGTTGCA	CCCTGGGTTGAATGGAATCC	45.0	55.0	57.9	58.2	1511-1512 bp	1.0	78.5
ycf4-cemA_p5	TGTTCGTTGGGGATTTCCTG	CCCTGGGTTGAATGGAATCC	50.0	55.0	58.1	58.2	1267-1268 bp	1.0	78.4