Hotspot

accD-psaI

LSC · 62906-63093 · 188 bp

Back to Amphicarpaea

Pi 0.0426

MarkerSeek score 72.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 8
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAATCGTACCGCGTAATCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.9	58.0	369-379 bp	1.0	72.4
accD-psaI_p2	TCAATCGTACCGCGTAATCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.9	57.9	375-385 bp	1.0	71.7
accD-psaI_p3	TCAATCGTACCGCGTAATCTT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.6	58.0	369-379 bp	1.0	70.1
accD-psaI_p4	TTCAATCGTACCGCGTAATCT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.6	58.0	370-380 bp	1.0	70.1
accD-psaI_p5	TCAATCGTACCGCGTAATCTT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.6	57.9	375-385 bp	1.0	69.5