Hotspot

atpA-trnR-UCU

LSC · 55801-55989 · 189 bp

Back to Amphicarpaea

Pi 0.0317

MarkerSeek score 70.80

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 6
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TTAACTGGGGAAGCAGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	301-326 bp	1.0	66.4
atpA-trnR-UCU_p2	GGAAGCAGAAGCCCTTTTGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.4	57.3	293-318 bp	1.0	65.0
atpA-trnR-UCU_p3	TTAACTGGGGAAGCAGAAGC	TGGATAGGACAGAGGTCTTCT	50.0	47.6	57.8	56.9	336-361 bp	1.0	63.4
atpA-trnR-UCU_p4	GGAAGCAGAAGCCCTTTTGA	TGGATAGGACAGAGGTCTTCT	50.0	47.6	58.4	56.9	328-353 bp	1.0	62.0
atpA-trnR-UCU_p5	TTAACTGGGGAAGCAGAAGC	GTTCAAATCCTATTGGACGCA	50.0	42.9	57.8	56.6	300-325 bp	1.0	60.8