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atpI-atpH

LSC · 51133-52231 · 1099 bp

Back to Amphicarpaea

Pi 0.0155

MarkerSeek score 68.10

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1099
variable_sites: 17
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	GCAGCAATAACAGAAGCAGC	50.0	50.0	57.3	58.1	1175-1179 bp	1.0	74.8
atpI-atpH_p2	TAGGTGAATCCATGGAAGGC	GCAGCAATAACAGAAGCAGC	50.0	50.0	57.0	58.1	1162-1166 bp	1.0	72.5
atpI-atpH_p3	GCTGCGGCTTATATAGGTGAA	GCAGCAATAACAGAAGCAGC	47.6	50.0	58.0	58.1	1175-1179 bp	1.0	72.4
atpI-atpH_p4	GCTGCGGCTTATATAGGTGA	AGGTCCAATAGAAGCAAGCC	50.0	50.0	57.3	57.6	1209-1213 bp	1.0	72.1
atpI-atpH_p5	GCTGCGGCTTATATAGGTGA	GTCCAATAGAAGCAAGCCCT	50.0	50.0	57.3	57.6	1207-1211 bp	1.0	72.1