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psbM-petN

LSC · 35742-36458 · 717 bp

Back to Amphicarpaea

Pi 0.0323

MarkerSeek score 73.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 23
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTTATTCT	TCTTGCTTGGGCTGCTTTAA	38.1	45.0	55.2	58.0	872-896 bp	1.0	52.3
psbM-petN_p2	TGCTACTGCACTGTTTATTCT	AGTAAGTCTTGCTTGGGCTG	38.1	50.0	55.2	57.8	878-902 bp	1.0	50.8
psbM-petN_p3	CGTAAAAACGGTAAGTCAAAGTGA	TCTTGCTTGGGCTGCTTTAA	37.5	45.0	58.0	58.0	821-845 bp	1.0	50.3
psbM-petN_p4	TGCTACTGCACTGTTTATTCTAGT	TCTTGCTTGGGCTGCTTTAA	37.5	45.0	58.0	58.0	872-896 bp	1.0	50.1
psbM-petN_p5	ACGTAAAAACGGTAAGTCAAAGT	TCTTGCTTGGGCTGCTTTAA	34.8	45.0	56.9	58.0	822-846 bp	1.0	50.1