Hotspot

trnG-GCC-psbZ

LSC · 28486-29159 · 674 bp

Back to Amphicarpaea

Pi 0.015
MarkerSeek score 68.50
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
674
variable_sites
10
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA CTTCTCCTGAAGGTTGGTCG 50.0 55.0 57.8 57.9 825-828 bp 1.0 79.7
trnG-GCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA TCTCCTGAAGGTTGGTCGAA 50.0 50.0 57.8 58.3 823-826 bp 1.0 78.1
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA TTCTCCTGAAGGTTGGTCGA 50.0 50.0 57.8 58.3 824-827 bp 1.0 78.1
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA CTCCTGAAGGTTGGTCGAAT 50.0 50.0 57.8 57.2 822-825 bp 1.0 74.2
trnG-GCC-psbZ_p5 CGGATAGCGGGAATCGAAC CTTCTCCTGAAGGTTGGTCG 57.9 55.0 58.2 57.9 847-850 bp 1.0 71.5