Hotspot

trnK-UUU-atpB

LSC · 8327-11479 · 3153 bp

Back to Amphicarpaea

Pi 0.0146

MarkerSeek score 54.20

Primer available yes

Label trnK-UUU-atpB

Feature Metadata

label_name: trnK-UUU-atpB
feature_type: igs
region: LSC
length_bp: 3153
variable_sites: 41
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-atpB_p1	TGGGCCGAGTTTAATTGCAA	CTACCTGGTGTTTTGCCTGT	45.0	50.0	58.4	58.0	350 bp	1.0	78.4
trnK-UUU-atpB_p2	TTGGGCCGAGTTTAATTGCA	CTACCTGGTGTTTTGCCTGT	45.0	50.0	58.4	58.0	351 bp	1.0	78.4
trnK-UUU-atpB_p3	TGGGCCGAGTTTAATTGCAA	TTTATGCGTTGGAGAGACCG	45.0	50.0	58.4	58.0	830 bp	1.0	78.3
trnK-UUU-atpB_p4	TTGGGCCGAGTTTAATTGCA	TTTATGCGTTGGAGAGACCG	45.0	50.0	58.4	58.0	831 bp	1.0	78.3
trnK-UUU-atpB_p5	TGGGCCGAGTTTAATTGCAA	GCTCTGACCGAGATCTTTGG	45.0	55.0	58.4	58.1	296 bp	1.0	78.3