Hotspot

trnH-GUG-psbA

LSC · 4161-4344 · 184 bp

Back to Amphicarpaea

Pi 0.0479

MarkerSeek score 69.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 8
pi: 0.0479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GGGCCCCAATTGGTAGAAAA	GTTATACAACGGCGGTCCTT	50.0	50.0	58.1	58.0	1292 bp	0.5	59.4
trnH-GUG-psbA_p2	GGGCCCCAATTGGTAGAAAA	ATATTCCGCTCCTGTTGCAG	50.0	50.0	58.1	58.0	1169 bp	0.5	59.2
trnH-GUG-psbA_p3	GGGCCCCAATTGGTAGAAAA	CTGTTGCATATTCCGCTCCT	50.0	50.0	58.1	58.0	1176 bp	0.5	59.2
trnH-GUG-psbA_p4	GGGCCCCAATTGGTAGAAAA	TCAGTGCTATGCATGGTTCC	50.0	50.0	58.1	58.0	975 bp	0.5	59.2
trnH-GUG-psbA_p5	GGGCCCCAATTGGTAGAAAA	CAGTGCTATGCATGGTTCCT	50.0	50.0	58.1	58.0	974 bp	0.5	59.2