Hotspot

clpP

LSC · 76167-78224 · 2058 bp

Back to Ampelopsis

Pi 0.00324

MarkerSeek score 43.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2058
variable_sites: 25
pi: 0.00324
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGA	45.0	40.0	57.1	54.9	2125-2183 bp	1.0	49.7
clpP_p2	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGAA	45.0	38.1	57.1	55.7	2125-2183 bp	1.0	48.3
clpP_p3	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGAAA	45.0	36.4	57.1	56.5	2125-2183 bp	1.0	46.3
clpP_p4	AAATCACGGATTTGCACGAA	ACGTTTCCATACCAAAGTGA	40.0	40.0	56.6	54.9	2128-2186 bp	1.0	45.9
clpP_p5	AATCACGGATTTGCACGAAA	ACGTTTCCATACCAAAGTGA	40.0	40.0	56.6	54.9	2127-2185 bp	1.0	45.9