Hotspot

rpl16-rps3

LSC · 87707-89008 · 1302 bp

Back to Ampelopsis

Pi 0.00453
MarkerSeek score 40.40
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1302
variable_sites
24
pi
0.00453
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CAAATGGGTCTGGGGTTGAA ATATCCTCTGGTGTCGAGGG 50.0 55.0 58.0 58.0 2296-2321 bp 1.0 81.9
rpl16-rps3_p2 TTCAAATGGGTCTGGGGTTG ATATCCTCTGGTGTCGAGGG 50.0 55.0 58.0 58.0 2298-2323 bp 1.0 81.8
rpl16-rps3_p3 ATGAGCGTATCGGCGATTAC ATATCCTCTGGTGTCGAGGG 50.0 55.0 58.0 58.0 2374-2399 bp 1.0 81.7
rpl16-rps3_p4 CAAATGGGTCTGGGGTTGAA CTCTGGTGTCGAGGGAATTG 50.0 55.0 58.0 58.0 2291-2316 bp 0.888889 76.7
rpl16-rps3_p5 TTCAAATGGGTCTGGGGTTG CTCTGGTGTCGAGGGAATTG 50.0 55.0 58.0 58.0 2293-2318 bp 0.888889 76.7