Hotspot

trnF-GAA-ndhJ

LSC · 53258-53967 · 710 bp

Back to Ampelopsis

Pi 0.00344

MarkerSeek score 53.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 11
pi: 0.00344
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TAATCATCCACGCCTGAAGC	55.0	50.0	58.0	58.0	880-890 bp	1.0	81.6
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	843-853 bp	1.0	81.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TAATCATCCACGCCTGAAGC	50.0	50.0	58.1	58.0	844-854 bp	1.0	81.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	807-817 bp	1.0	81.0
trnF-GAA-ndhJ_p5	ATGATGCATCGGGAATAGCC	TAATCATCCACGCCTGAAGC	50.0	50.0	57.9	58.0	900-910 bp	1.0	80.6