Hotspot

psbC-trnS-UGA

LSC · 39361-39599 · 239 bp

Back to Ampelopsis

Pi 0.0121

MarkerSeek score 52.10

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 13
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCGTAGGTCATTTGTGGCAC	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.2	58.1	424-426 bp	1.0	81.9
psbC-trnS-UGA_p2	TCGTAGGTCATTTGTGGCAC	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.2	58.1	435-437 bp	1.0	81.2
psbC-trnS-UGA_p3	TCGTAGGTCATTTGTGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	370-372 bp	1.0	81.2
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	387-389 bp	1.0	80.8
psbC-trnS-UGA_p5	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	386-388 bp	1.0	80.3