Hotspot

trnE-UUC-psbD

LSC · 34658-36929 · 2272 bp

Back to Ampelopsis

Pi 0.0034

MarkerSeek score 44.60

Primer available yes

Label trnE-UUC-psbD

Feature Metadata

label_name: trnE-UUC-psbD
feature_type: igs
region: LSC
length_bp: 2272
variable_sites: 32
pi: 0.0034
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbD_p1	TCCTTTCTTCCTACCCCCAG	TCGCTTTCGTCTTTGGTGAA	55.0	45.0	58.0	58.1	2374-2407 bp	1.0	81.6
trnE-UUC-psbD_p2	TCCTTTCTTCCTACCCCCAG	ATCGCTTTCGTCTTTGGTGA	55.0	45.0	58.0	57.8	2375-2408 bp	1.0	80.8
trnE-UUC-psbD_p3	GCTGCCTCCTTGAAAGAGAG	TCGCTTTCGTCTTTGGTGAA	55.0	45.0	58.3	58.1	2338-2371 bp	1.0	79.8
trnE-UUC-psbD_p4	TGTCCTGAACCACTAGACGA	TCGCTTTCGTCTTTGGTGAA	50.0	45.0	57.7	58.1	2317-2350 bp	1.0	79.7
trnE-UUC-psbD_p5	GCTGCCTCCTTGAAAGAGAG	ATCGCTTTCGTCTTTGGTGA	55.0	45.0	58.3	57.8	2339-2372 bp	1.0	79.0