Hotspot

psbM-trnD-GUC

LSC · 32746-33928 · 1183 bp

Back to Ampelopsis

Pi 0.00475

MarkerSeek score 59.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 22
pi: 0.00475
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TGGGCTATGGCTATACACCT	45.0	50.0	55.8	57.6	1277-1351 bp	1.0	64.2
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	GTGGGCTATGGCTATACACC	45.0	55.0	55.8	57.5	1278-1352 bp	1.0	63.7
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1238-1312 bp	1.0	63.7
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	TGGGCTATGGCTATACACCT	40.9	50.0	57.4	57.6	1284-1358 bp	1.0	61.0
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	GTGGGCTATGGCTATACACC	40.9	55.0	57.4	57.5	1285-1359 bp	1.0	60.5