Hotspot

rpoB-trnC-GCA

LSC · 29111-30393 · 1283 bp

Back to Ampelopsis

Pi 0.00297

MarkerSeek score 55.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1283
variable_sites: 15
pi: 0.00297
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTCCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.2	58.1	1170-1373 bp	1.0	82.5
rpoB-trnC-GCA_p2	CATTCCCTCATTTCCACCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.2	58.4	1152-1355 bp	1.0	80.3
rpoB-trnC-GCA_p3	ACATTCCCTCATTTCCACCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1171-1374 bp	1.0	80.0
rpoB-trnC-GCA_p4	GTTCTTCTGTCAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.4	58.1	1246-1449 bp	1.0	79.8
rpoB-trnC-GCA_p5	AGTTCTTCTGTCAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.4	58.1	1247-1450 bp	1.0	79.7