Hotspot

atpF-atpI

LSC · 15210-16833 · 1624 bp

Back to Ampelopsis

Pi 0.00216

MarkerSeek score 55.20

Primer available yes

Label atpF-atpI

Feature Metadata

label_name: atpF-atpI
feature_type: igs
region: LSC
length_bp: 1624
variable_sites: 15
pi: 0.00216
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpI_p1	CAGTGACCCAAGGAAACGAA	CGAATCCATGGAGGGTCATC	50.0	55.0	58.0	57.8	1688-1691 bp	1.0	79.7
atpF-atpI_p2	CAGTGACCCAAGGAAACGAA	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	1719-1722 bp	1.0	76.9
atpF-atpI_p3	TATTAAACCCGAAACCCCCG	CGAATCCATGGAGGGTCATC	50.0	55.0	57.6	57.8	1717-1720 bp	1.0	76.6
atpF-atpI_p4	AGTGACCCAAGGAAACGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.3	57.8	1687-1690 bp	1.0	74.2
atpF-atpI_p5	TATTAAACCCGAAACCCCCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.6	58.5	1748-1751 bp	1.0	73.9