Hotspot

trnK-UUU-rps16

LSC · 4374-5297 · 924 bp

Back to Ampelopsis

Pi 0.00623

MarkerSeek score 50.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 25
pi: 0.00623
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.2	57.9	852-1014 bp	1.0	68.2
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.2	57.9	851-1013 bp	1.0	68.2
trnK-UUU-rps16_p3	CAGTCGCGGTCTTACAAACT	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.2	57.9	936-1098 bp	1.0	67.6
trnK-UUU-rps16_p4	TCAGTCGCGGTCTTACAAAC	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.2	57.9	937-1099 bp	1.0	67.6
trnK-UUU-rps16_p5	TCTAGCCGCACTTAAAAGCC	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.3	57.9	866-1028 bp	1.0	67.4