Hotspot

rps15-ycf1

SSC · 130911-131331 · 421 bp

Back to Ampelocera

Pi 0.0515

MarkerSeek score 79.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 421
variable_sites: 42
pi: 0.0515
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTACGTAGGCCCTTCTGTGA	TTTATCCCCCAGACGTCGTA	50.0	50.0	58.1	57.9	855-888 bp	1.0	87.2
rps15-ycf1_p2	AACACCATGCATTGATGGGT	TTTATCCCCCAGACGTCGTA	45.0	50.0	58.1	57.9	1149-1187 bp	1.0	85.8
rps15-ycf1_p3	GAGGTCTTGTCTTGGAGCTG	TTTATCCCCCAGACGTCGTA	55.0	50.0	57.9	57.9	1104-1142 bp	1.0	85.8
rps15-ycf1_p4	TCCCAACGCGTTACATAAGG	TTTATCCCCCAGACGTCGTA	50.0	50.0	58.0	57.9	1286-1324 bp	1.0	85.4
rps15-ycf1_p5	TCCTCGGTGTAAATAGCCCA	TTTATCCCCCAGACGTCGTA	50.0	50.0	58.1	57.9	1224-1262 bp	1.0	84.8