Hotspot

atpB-rbcL

LSC · 59091-59885 · 795 bp

Back to Ampelocera

Pi 0.0108
MarkerSeek score 63.50
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
795
variable_sites
15
pi
0.0108
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CATCCAGTACCGGACCAATG CACCAGCTTTGAATCCAACAC 55.0 47.6 58.0 58.0 932-956 bp 1.0 74.0
atpB-rbcL_p2 CATCCAGTACCGGACCAATG ACCAGCTTTGAATCCAACACT 55.0 42.9 58.0 58.1 931-955 bp 1.0 73.8
atpB-rbcL_p3 CGGTGGAAAAGCTACATCCA CACCAGCTTTGAATCCAACAC 50.0 47.6 57.9 58.0 946-970 bp 1.0 73.5
atpB-rbcL_p4 ATCCAGTACCGGACCAATGA CACCAGCTTTGAATCCAACAC 50.0 47.6 58.1 58.0 931-955 bp 1.0 73.4
atpB-rbcL_p5 TCCAGTACCGGACCAATGAT CACCAGCTTTGAATCCAACAC 50.0 47.6 58.1 58.0 930-954 bp 1.0 73.4