Hotspot

psbZ-trnG-GCC

LSC · 39420-40015 · 596 bp

Back to Ampelocera

Pi 0.0175
MarkerSeek score 67.00
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
596
variable_sites
19
pi
0.0175
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG AATCGAACCCGCATCTTCTC 55.0 50.0 58.0 58.1 761-811 bp 1.0 83.7
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG GAATCGAACCCGCATCTTCT 55.0 50.0 58.0 58.1 762-812 bp 1.0 83.7
psbZ-trnG-GCC_p3 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 764-814 bp 1.0 82.2
psbZ-trnG-GCC_p4 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 765-815 bp 1.0 82.2
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 762-812 bp 1.0 81.8