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atpH-atpI

LSC · 15564-16837 · 1274 bp

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Pi 0.00863

MarkerSeek score 66.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1274
variable_sites: 18
pi: 0.00863
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATCCCTTCTACAGCTT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.0	57.9	1434-1451 bp	1.0	81.2
atpH-atpI_p2	CGCAATCCCTTCTACAGCTT	TAGCCGCCGCTTATATAGGA	50.0	50.0	58.0	57.9	1421-1438 bp	1.0	80.8
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.8	57.9	1354-1371 bp	1.0	80.2
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.8	57.9	1341-1358 bp	1.0	79.8
atpH-atpI_p5	AATCCCTTCTACAGCTTGGC	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.6	57.9	1431-1448 bp	1.0	78.1