Hotspot

atpF-atpH

LSC · 14865-15317 · 453 bp

Back to Ampelocera

Pi 0.00442

MarkerSeek score 64.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 453
variable_sites: 4
pi: 0.00442
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTTAACCCGAAACTCCCGG	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.9	58.0	562-650 bp	1.0	82.8
atpF-atpH_p2	GCCAAAAACCCAAGGAAAGG	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.7	58.0	536-624 bp	1.0	81.9
atpF-atpH_p3	GGCCAAAAACCCAAGGAAAG	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.7	58.0	537-625 bp	1.0	81.9
atpF-atpH_p4	ATTTAACCCGAAACTCCCGG	TGGACTGGTTGTGGCATTAG	50.0	50.0	57.9	57.8	567-655 bp	1.0	81.2
atpF-atpH_p5	GCCAAAAACCCAAGGAAAGG	TGGACTGGTTGTGGCATTAG	50.0	50.0	57.7	57.8	541-629 bp	1.0	80.3