Hotspot

trnS-GCU-trnG-GCC

LSC · 9597-10346 · 750 bp

Back to Ampelocera

Pi 0.0328
MarkerSeek score 68.70
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
750
variable_sites
38
pi
0.0328
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.89
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.0 58.0 1455-1534 bp 1.0 86.2
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.0 58.0 1454-1533 bp 1.0 86.2
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA CGTTAGCTTGGAAGGCTAGG 50.0 55.0 58.0 58.1 1452-1531 bp 1.0 86.0
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC CGTTAGCTTGGAAGGCTAGG 50.0 55.0 58.0 58.1 1451-1530 bp 1.0 86.0
trnS-GCU-trnG-GCC_p5 ATGATCCGGGTCGTAATCCT CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.0 58.0 1676-1755 bp 1.0 85.9