Hotspot

rps16-trnQ-UUG

LSC · 7075-8154 · 1080 bp

Back to Ampelocera

Pi 0.0306

MarkerSeek score 73.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1080
variable_sites: 53
pi: 0.0306
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1087-1224 bp	1.0	86.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1070-1207 bp	1.0	86.0
rps16-trnQ-UUG_p3	AACGGATCGTGTCCTTCAAG	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	1117-1254 bp	1.0	85.5
rps16-trnQ-UUG_p4	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1088-1225 bp	1.0	85.4
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	1100-1237 bp	1.0	85.1