Hotspot

matK-rps16

LSC · 3802-5876 · 2075 bp

Back to Ampelocera

Pi 0.0185

MarkerSeek score 65.10

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 2075
variable_sites: 58
pi: 0.0185
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GCGGGTATAGGAAGTCATGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	56.8	58.3	2005-2348 bp	1.0	74.0
matK-rps16_p2	AGCGGGTATAGGAAGTCATGT	TTCCTTGAAAAAGGCGCTCA	47.6	45.0	58.3	58.3	2006-2349 bp	1.0	73.1
matK-rps16_p3	GCGGGTATAGGAAGTCATGT	GCTCAGCCTACAAGAACTGT	50.0	50.0	56.8	57.5	1990-2333 bp	1.0	72.8
matK-rps16_p4	GCGGGTATAGGAAGTCATGT	TCAAAGAAGGCAGGGGTTTT	50.0	45.0	56.8	57.5	1960-2303 bp	1.0	72.8
matK-rps16_p5	GCGGGTATAGGAAGTCATGT	TTCAAAGAAGGCAGGGGTTT	50.0	45.0	56.8	57.5	1961-2304 bp	1.0	72.8