Hotspot

petD

LSC · 75525-76756 · 1232 bp

Back to Ampelocalamus

Pi 0.00774

MarkerSeek score 45.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1232
variable_sites: 51
pi: 0.00774
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCCGGTAATAGGATCGCCTT	GGAAGAGGGCACTAGTTTCG	50.0	55.0	58.0	58.0	2123-2143 bp	1.0	82.8
petD_p2	TGGGTGTATTGACTGCATCG	GGAAGAGGGCACTAGTTTCG	50.0	55.0	58.0	58.0	2226-2246 bp	1.0	82.6
petD_p3	GGGTGTATTGACTGCATCGT	GGAAGAGGGCACTAGTTTCG	50.0	55.0	58.0	58.0	2225-2245 bp	1.0	82.6
petD_p4	CAGTTCATCGATGGTCAGCA	GGAAGAGGGCACTAGTTTCG	50.0	55.0	58.0	58.0	2346-2366 bp	1.0	82.5
petD_p5	TCCGGTAATAGGATCGCCTT	TGCTGTATCCATGCCTGTTC	50.0	50.0	58.0	58.0	2303-2323 bp	1.0	82.5